ELISA/assay

Human Integrin alphaM, ITG/CD11b ELISA Kit

MBS702254

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Integrin alphaM, ITG/CD11b ELISA Kit

integrin, alpha M (complement component 3 receptor 3 subunit)

Human Integrin alphaM; ITG/CD11b ELISA Kit; CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170) ; integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit; integrin; alpha M (complement component 3 receptor 3 subunit)

integrin alpha-M isoform 2; Integrin alpha-M; integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; integrin, alpha M (complement component 3 receptor 3 subunit); CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b

ITGAM

ITGAM;ITGAM;CR3A;MO1A;CD11B;MAC-1;MAC1A;SLEB6;CD11B;CR3A

ITAM_HUMAN

人类

1152

This assay has high sensitivity and excellent specificity for detection of human ITG/CD11b. No significant cross-reactivity or interference between human ITG/CD11b and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.195 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITG/CD11b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITG/CD11b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITG/CD11b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITG/CD11b bound in the initial step. The color development is stopped and the intensity of the color is measured.

88501734

NP_000623.2

NM_000632.3

P11215

127,179 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028)

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Function: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800