ELISA/assay

人类血管紧张素, aGT酶联免疫吸附试剂盒

MBS702389

48-Strip-Wells

565美元

酶联免疫吸附试剂盒

人类血管紧张素, aGT酶联免疫吸附试剂盒

angiotensinogen (serpin peptidase inhibitor, clade A, member 8)

Human angiotensinogen (aGT) ELISA Kit; ANHU; FLJ92595; FLJ97926; SERPINA8; alpha-1 antiproteinase; antitrypsin; angiotensin I; angiotensin II; angiotensinogen; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; angiotensinogen (serpin peptidase inhibitor; clade A; member 8)

angiotensinogen preproprotein; Angiotensinogen; angiotensinogen; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Serpin A8Cleaved into the following 8 chains:Angiotensin-1Alternative name(s):Angiotensin 1-10; Angiotensin I; Ang I

AGT

AGT; AGT; ANHU; SERPINA8; SERPINA8; Ang I; Ang II; Ang III; Ang IV

ANGT_HUMAN

人类

485

This assay has high sensitivity and excellent specificity for detection of human aGT. No significant cross-reactivity or interference between human aGT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, cell culture supernates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: The minimum detectable dose of human aGT is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for aGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any aGT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for aGT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of aGT bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557287

NP_000020.1

NM_000029.3

P01019

53,154 Da

ACE Inhibitor Pathway (198763); Adipogenesis Pathway (198832); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolism Pathway (477135)

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]

Function: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.3 PublicationsManual assertion based on experiment in:Ref.11

48-Strip-Wells

565美元

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150