产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Human 8-oxoguanine DNA glycosydase, hOGG1 ELISA Kit
目录 :
MBS702793
规格 :
48-Strip-Wells
价格 :
590美元
更多信息或购买 :
产品信息
目录号 :
MBS702793
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Human 8-oxoguanine DNA glycosydase, hOGG1 ELISA Kit
产品简称 :
8-oxoguanine DNA glycosylase
产品名称同义词 :
Human 8-oxoguanine DNA glycosydase (hOGG1) ELISA kit; HMMH; HOGG1; MUTM; OGH1; 8-hydroxyguanine DNA glycosylase; 8-oxoguanine DNA-glycosylase 1; AP lyase; DNA-apurinic or apyrimidinic site lyase; 8-oxoguanine DNA glycosylase
其他名称 :
N-glycosylase/DNA lyase isoform 1a; N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase; 8-oxoguanine DNA glycosylase
产品基因名称 :
OGG1
其他基因名称 :
OGG1;OGG1;HMMH;MUTM;OGH1;HOGG1;MMH;MUTM;OGH1;ap裂解酶
UniProt数据库进入名 :
OGG1_HUMAN
反应物种 :
人类
序列长度 :
345
特异性 :
This assay has high sensitivity and excellent specificity for detection of human hOGG1. No significant cross-reactivity or interference between human hOGG1 and analogues was observed.
储存稳定性 :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
其它信息1 :
Samples: Serum, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
产品描述 :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for hOGG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any hOGG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for hOGG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of hOGG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI GI登录号 :
4505495
NCBI登录号 :
NP_002533.1
NCBI基因登录号 :
NM_002542.5
UniProt数据库登录号 :
O15527
NCBI分子量 :
38,782 Da
NCBI信号通路 :
Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)
NCBI总结 :
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
UniProt数据库总结 :
Function: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. Catalytic activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
尺寸1 :
48-Strip-Wells
价格1 :
590美元
尺寸2 :
96-Strip-Wells
价格2 :
850
size3 :
5x96-Strip-Wells
价格3 :
2880
size4 :
10x96-Strip-Wells
price4 :
5440
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。