产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
人类p53/肿瘤蛋白, p53/TP53酶联免疫吸附试剂盒
目录 :
MBS703562
规格 :
96 Strip Wells
价格 :
760美元
更多信息或购买 :
产品信息
目录号 :
MBS703562
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
人类p53/肿瘤蛋白, p53/TP53酶联免疫吸附试剂盒
产品简称 :
肿瘤蛋白p53
产品名称同义词 :
Human p53/tumor protein; p53/TP53 ELISA Kit; FLJ92943; LFS1; TRP53; p53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; tumor protein p53
其他名称 :
cellular tumor antigen p53 isoform a; Cellular tumor antigen p53; cellular tumor antigen p53; antigen NY-CO-13; tumor protein 53; phosphoprotein p53; p53 tumor suppressor; mutant tumor protein 53; transformation-related protein 53; tumor protein p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
产品基因名称 :
TP53
其他基因名称 :
TP53;TP53;P53;BCC7;LFS1;TRP53;P53
UniProt数据库进入名 :
P53_HUMAN
反应物种 :
人类
序列长度 :
393
特异性 :
This assay has high sensitivity and excellent specificity for detection of Human TP53. No significant cross-reactivity or interference between Human TP53 and analogues was observed.
储存稳定性 :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
其它信息1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates. Detection Range: 9.38 pg/ml-600 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 2.34 pg/ml
其他信息2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis
产品描述 :
Introduction: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. p53 is important in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. As such, p53 has been described as "the guardian of the genome", the "guardian angel gene", and the "master watchman", referring to its role in conserving stability by preventing genome mutation.The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of proline residues in the protein, which slow its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fish. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to p53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for p53 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain p53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of p53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI GI登录号 :
120407068
NCBI登录号 :
NP_000537.3
NCBI基因登录号 :
NM_000546.5
UniProt数据库登录号 :
P04637
NCBI分子量 :
43,653 Da
NCBI信号通路 :
AMPK Signaling Pathway 198868!!Activation Of BH3-only Proteins Pathway 105658!!Activation Of NOXA And Translocation To Mitochondria Pathway 105660!!Activation Of PUMA And Translocation To Mitochondria Pathway 105661!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060
NCBI总结 :
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
UniProt数据库总结 :
Function: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.11 PublicationsManual assertion based on experiment in:Ref.34. Cofactor: Binds 1 zinc ion per subunit.
规格 :
96 Strip Wells
价格 :
760美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。