产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
人类环氧合酶-2, COX-2酶联免疫吸附试剂盒
目录 :
MBS703694
规格 :
48-Strip-Wells
价格 :
565美元
更多信息或购买 :
产品信息
目录号 :
MBS703694
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
人类环氧合酶-2, COX-2酶联免疫吸附试剂盒
产品简称 :
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
产品名称同义词 :
Human cyclooxygenase-2; COX-2 ELISA Kit; COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; cyclooxygenase 2b; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
其他名称 :
prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2
产品基因名称 :
PTGS2
其他基因名称 :
PTGS2; PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2; COX-2; PGH synthase 2; PGHS-2
UniProt数据库进入名 :
PGH2_HUMAN
反应物种 :
人类
序列长度 :
604
特异性 :
This assay has high sensitivity and excellent specificity for detection of Human PTGS2. No significant cross-reactivity or interference between Human PTGS2 and analogues was observed.
储存稳定性 :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
其它信息1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.31 ng/ml
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Fatty acid biosynthesis
产品描述 :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COX-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COX-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COX-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI GI登录号 :
4506265
NCBI登录号 :
NP_000954.1
NCBI基因登录号 :
NM_000963.3
UniProt数据库登录号 :
P35354
NCBI分子量 :
68,996 Da
NCBI信号通路 :
Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); C-MYB Transcription Factor Network Pathway (138073); C20 Prostanoid Biosynthesis Pathway (908107); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000)
NCBI总结 :
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
UniProt数据库总结 :
Function: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.1 PublicationManual assertion based on experiment in:Ref.13. Catalytic activity: Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O.1 PublicationManual assertion based on experiment in:Ref.13. Cofactor: Binds 1 heme B (iron-protoporphyrin IX) group per subunit
尺寸1 :
48-Strip-Wells
价格1 :
565美元
尺寸2 :
96-Strip-Wells
价格2 :
810
size3 :
5x96-Strip-Wells
价格3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。