ELISA/assay

Human decay-accelerating factor, DAF/CD55 ELISA Kit

MBS703778

48-Strip-Wells

565美元

酶联免疫吸附试剂盒

Human decay-accelerating factor, DAF/CD55 ELISA Kit

CD55 molecule, decay accelerating factor for complement (Cromer blood group)

Human decay-accelerating factor (DAF/CD55) ELISA Kit; RP11-357P18.1; CR; CROM; DAF; TC; CD55 antigen; decay accelerating factor for complement; CD55 molecule; decay accelerating factor for complement (Cromer blood group)

complement decay-accelerating factor isoform 1 preproprotein; Complement decay-accelerating factor; complement decay-accelerating factor; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD_antigen: CD55

CD55

CD55;CD55;CR;TC;DAF;CROM;CR;DAF

DAF_HUMAN

人类

381

This assay has high sensitivity and excellent specificity for detection of human DAF/CD55. No significant cross-reactivity or interference between human DAF/CD55 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human DAF/CD55 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAF/CD55 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAF/CD55 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAF/CD55 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAF/CD55 bound in the initial step. The color development is stopped and the intensity of the color is measured.

10835143

NP_000565.1

NM_000574.4

P08174

41,400 Da

Class B/2 (Secretin Family Receptors) Pathway (106378); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); GPCR Ligand Binding Pathway (161020); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Innate Immune System Pathway (106387)

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

Function: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.1 PublicationManual assertion based on experiment in:Ref.15

48-Strip-Wells

565美元

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150