蛋白

重组人类转录因子p65蛋白

MBS717110

0.05毫克(大肠杆菌)

190美元

重组蛋白

重组人类转录因子p65蛋白

转录因子p65

Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

transcription factor p65 isoform 2; Transcription factor p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

NFKB3

RELA;RELA;p65;NFKB3;NFKB3

TF65_HUMAN

大肠杆菌

1-210

548

MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCE
GRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVT
KDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLG
IQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNA
VRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELK
ICRVNRNSGSCLGGD

Greater than 90% as determined by SDS-PAGE.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

免疫学

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with mbers of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells

Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.Ruben S.M., Dillon P.J., Schreck R., Henkel T., Chen C.-H., Maher M., Baeuerle P.A., Rosen C.A.Science 251:1490-1493(1991) Genomic organization of the gene encoding the p65 subunit of NF-kappa B multiple variants of the p65 protein may be generated by alternative splicing.Deloukas P., van Loon A.P.G.M.Hum. Mol. Genet. 2:1895-1900(1993) An alternatively spliced transcript, p65 delta 2, of the gene encoding the p65 subunit of the transcription factor NF-kappa B.Lyle R., Valleley E.M., Sharpe P.T., Hewitt J.E.Gene 138:265-266(1994) SeattleSNPs variation discovery resourceFunctional characterization of the NF-kappa B p65 transcriptional activator and an alternatively spliced derivative.Ruben S.M., Narayanan R., Klement J.F., Chen C.-H., Rosen C.A.Mol. Cell. Biol. 12:444-454(1992) A novel complex between the p65 subunit of NF-kappa B and c-Rel binds to a DNA element involved in the phorbol ester induction of the human urokinase gene.Hansen S.K., Nerlov C., Zabel U., Verde P., Johnsen M., Baeuerle P.A., Blasi F.EMBO J. 11:205-213(1992) I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding.Ganchi P.A., Sun S.C., Greene W.C., Ballard D.W.Mol. Biol. Cell 3:1339-1352(1992) Activation of multiple NF-kappa B/Rel DNA-binding complexes by tumor necrosis factor.Beg A.A., Baldwin A.S. Jr.Oncogene 9:1487-1492(1994) Regulation of intercellular adhesion molecule-1 gene by tumor necrosis factor-alpha is mediated by the nuclear factor-kappaB heterodimers p65/p65 and p65/c-Rel in the absence of p50.Aoudjit F., Brochu N., Belanger B., Stratowa C., Hiscott J., Audette M.Cell Growth Differ. 8:335-342(1997) A new member of the IkappaB protein family, IkappaB epsilon, inhibits RelA (p65) -mediated NF-kappaB transcription.Li Z., Nabel G.J.Mol. Cell. Biol. 17:6184-6190(1997) IKAP is a scaffold protein of the IkappaB kinase complex.Cohen L., Henzel W.J., Baeuerle P.A.Nature 395:292-296(1998) IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain.Sakurai H., Chiba H., Miyoshi H., Sugita T., Toriumi W.J. Biol. Chem. 274:30353-30356(1999) NF-kappaB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2.Yang J.-P., Hori M., Takahashi N., Kawabe T., Kato H., Okamoto T.Oncogene 18:5177-5186(1999) Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers.Schulte R., Grassl G.A., Preger S., Fessele S., Jacobi C.A., Schaller M., Nelson P.J., Autenrieth I.B.FASEB J. 14:1471-1484(2000) Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats.Tetsuka T., Uranishi H., Imai H., Ono T., Sonta S., Takahashi N., Asamitsu K., Okamoto T.J. Biol. Chem. 275:4383-4390(2000) Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II.Wang D., Westerheide S.D., Hanson J.L., Baldwin A.S. Jr.J. Biol. Chem. 275:32592-32597(2000) The tumor suppressor protein menin interacts with NF-kappaB proteins and inhibits NF-kappaB-mediated transactivation.Heppner C., Bilimoria K.Y., Agarwal S.K., Kester M., Whitty L.J., Guru S.C., Chandrasekharappa S.C., Collins F.S., Spiegel A.M., Marx S.J., Burns A.L.Oncogene 20:4917-4925(2001) Duration of nuclear NF-kappaB action regulated by reversible acetylation.Chen L.F., Fischle W., Verdin E., Greene W.C.Science 293:1653-1657(2001) A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis.Higashitsuji H., Higashitsuji H., Nagao T., Nonoguchi K., Fujii S., Itoh K., Fujita J.Cancer Cell 2:335-346(2002) Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB.Chen L.F., Mu Y., Greene W.C.EMBO J. 21:6539-6548(2002) The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1.Zhong H., May M.J., Jimi E., Ghosh S.Mol. Cell 9:625-636(2002) Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1) .Vermeulen L., De Wilde G., Van Damme P., Vanden Berghe W., Haegeman G.EMBO J. 22:1313-1324(2003) Post-activation turn-off of NF-kappa B-dependent transcription is regulated by acetylation of p65.Kiernan R., Bres V., Ng R.W., Coudart M.-P., El Messaoudi S., Sardet C., Jin D.-Y., Emiliani S., Benkirane M.J. Biol. Chem. 278:2758-2766(2003) RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.Asamitsu K., Tetsuka T., Kanazawa S., Okamoto T.J. Biol. Chem. 278:26879-26887(2003) Glycogen synthase kinase-3 beta regulates NF-kappa B1/p105 stability.Demarchi F., Bertoli C., Sandy P., Schneider C.J. Biol. Chem. 278:39583-39590(2003) Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA.Ryo A., Suizu F., Yoshida Y., Perrem K., Liou Y.C., Wulf G., Rottapel R., Yamaoka S., Lu K.P.Mol. Cell 12:1413-1426(2003) Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.Zhang J., Xu L.-G., Han K.-J., Shu H.-B.J. Biol. Chem. 279:17819-17825(2004) Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation.Yeh P.Y., Yeh K.H., Chuang S.E., Song Y.C., Cheng A.L.J. Biol. Chem. 279:26143-26148(2004) Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response.Saccani S., Marazzi I., Beg A.A., Natoli G.J. Exp. Med. 200:107-113(2004) Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways.Gao H., Sun Y., Wu Y., Luan B., Wang Y., Qu B., Pei G.Mol. Cell 14:303-317(2004) The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis.Garkavtsev I., Kozin S.V., Chernova O., Xu L., Winkler F., Brown E., Barnett G.H., Jain R.K.Nature 428:328-332(2004) Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor.Rocha S., Garrett M.D., Campbell K.J., Schumm K., Perkins N.D.EMBO J. 24:1157-1169(2005) IKKbeta phosphorylates p65 at S468 in transactivation domain 2.Schwabe R.F., Sakurai H.FASEB J. 19:1758-1760(2005) cis-acting, element-specific transcriptional activity of differentially phosphorylated nuclear factor-kappa B.Anrather J., Racchumi G., Iadecola C.J. Biol. Chem. 280:244-252(2005) COMMD proteins, a novel family of structural and functional homologs of MURR1.Burstein E., Hoberg J.E., Wilkinson A.S., Rumble J.M., Csomos R.A., Komarck C.M., Maine G.N., Wilkinson J.C., Mayo M.W., Duckett C.S.J. Biol. Chem. 280:22222-22232(2005) NF-kappaB RelA phosphorylation regulates RelA acetylation.Chen L.F., Williams S.A., Mu Y., Nakano H., Duerr J.M., Buckbinder L., Greene W.C.Mol. Cell. Biol. 25:7966-7975(2005) Foxp3 interacts with nuclear factor of activated T cells and NF-kappa B to repress cytokine gene expression and effector functions of T helper cells.Bettelli E., Dastrange M., Oukka M.Proc. Natl. Acad. Sci. U.S.A. 102:5138-5143(2005) Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B.Reimers K., Buchholz K., Werchau H.Virology 331:260-268(2005) Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1 implications for tumor progression and metastasis.Emdad L., Sarkar D., Su Z.-Z., Randolph A., Boukerche H., Valerie K., Fisher P.B.Cancer Res. 66:1509-1516(2006) Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB.Tzimas C., Michailidou G., Arsenakis M., Kieff E., Mosialos G., Hatzivassiliou E.G.Cell. Signal. 18:83-92(2006) Inducible phosphorylation of NF-kappa B p65 at serine 468 by T cell costimulation is mediated by IKK epsilon.Mattioli I., Geng H., Sebald A., Hodel M., Bucher C., Kracht M., Schmitz M.L.J. Biol. Chem. 281:6175-6183(2006) Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis.Liu Y., Smith P.W., Jones D.R.Mol. Cell. Biol. 26:8683-8696(2006) Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.Miao F., Li S., Chavez V., Lanting L., Natarajan R.Mol. Endocrinol. 20:1562-1573(2006) IL-1 receptor-associated kinase 1 is critical for latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation.Song Y.J., Jen K.Y., Soni V., Kieff E., Cahir-McFarland E.Proc. Natl. Acad. Sci. U.S.A. 103:2689-2694(2006) Nine-amino-acid transactivation domain establishment and prediction utilities.Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.Genomics 89:756-768(2007) UXT is a novel and essential cofactor in the NF-kappaB transcriptional enhanceosome.Sun S., Tang Y., Lou X., Zhu L., Yang K., Zhang B., Shi H., Wang C.J. Cell Biol. 178:231-244(2007) The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its N-terminal fragment.Chae J.J., Wood G., Richard K., Jaffe H., Colburn N.T., Masters S.L., Gumucio D.L., Shoham N.G., Kastner D.L.Blood 112:1794-1803(2008) Functional characterization of the atopy-associated gene PHF11.Clarke E., Rahman N., Page N., Rolph M.S., Stewart G.J., Jones G.J.J. Allergy Clin. Immunol. 121:1148-1154(2008) AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65.Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.J. Biol. Chem. 283:7834-7843(2008) The DEAD-box RNA helicase DDX1 interacts with RelA and enhances nuclear factor kappaB-mediated transcription.Ishaq M., Ma L., Wu X., Mu Y., Pan J., Hu J., Hu T., Fu Q., Guo D.J. Cell. Biochem. 106:296-305(2009) Brd4 coactivates transcriptional activation of NF-kappaB via specific binding to acetylated RelA.Huang B., Yang X.D., Zhou M.M., Ozato K., Chen L.F.Mol. Cell. Biol. 29:1375-1387(2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2009) A novel LZAP-binding protein, NLBP, inhibits cell invasion.Kwon J., Cho H.J., Han S.H., No J.G., Kwon J.Y., Kim H.J. Biol. Chem. 285:12232-12240(2010) SIRT2 regulates NF-kappaB dependent gene expression through deacetylation of p65 Lys310.Rothgiesser K.M., Erener S., Waibel S., Luscher B., Hottiger M.O.J. Cell Sci. 123:4251-4258(2010) Zinc finger protein Gfi1 controls the endotoxin-mediated Toll-like receptor inflammatory response by antagonizing NF-kappaB p65.Sharif-Askari E., Vassen L., Kosan C., Khandanpour C., Gaudreau M.C., Heyd F., Okayama T., Jin J., Rojas M.E., Grimes H.L., Zeng H., Moroy T.Mol. Cell. Biol. 30:3929-3942(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) NF-kappaB repression by PIAS3 mediated RelA SUMOylation.Liu Y., Bridges R., Wortham A., Kulesz-Martin M.PLoS ONE 7:E37636-E37636(2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) C11orf95-RELA fusions drive oncogenic NF-kappaB signalling in ependymoma.Parker M., Mohankumar K.M., Punchihewa C., Weinlich R., Dalton J.D., Li Y., Lee R., Tatevossian R.G., Phoenix T.N., Thiruvenkatam R., White E., Tang B., Orisme W., Gupta K., Rusch M., Chen X., Li Y., Nagahawhatte P. , Hedlund E., Finkelstein D., Wu G., Shurtleff S., Easton J., Boggs K., Yergeau D., Vadodaria B., Mulder H.L., Becksfort J., Becksford J., Gupta P., Huether R., Ma J., Song G., Gajjar A., Merchant T., Boop F., Smith A.A., Ding L., Lu C., Ochoa K., Zhao D., Fulton R.S., Fulton L.L., Mardis E.R., Wilson R.K., Downing J.R., Green D.R., Zhang J., Ellison D.W., Gilbertson R.J.Nature 506:451-455(2014) Structure of an IkappaBalpha/NF-kappaB complex.Jacobs M.D., Harrison S.C.Cell 95:749-758(1998) +Additional computationally mapped references. p>Provides general information on the entry.

223468681

NP_001138610.1

NM_001145138.1

Q04206

51kD

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (1269236); Activation Of NF-kappaB In B Cells Pathway (1269186); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505)

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex. Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding. Biological Process: acetaldehyde metabolic process; activation of NF-kappaB transcription factor; aging; cellular defense response; cytokine and chemokine mediated signaling pathway; defense response to virus; hair follicle development; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; liver development; membrane protein intracellular domain proteolysis; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to amino acid stimulus; response to cAMP; response to cobalamin; response to drug; response to insulin stimulus; response to morphine; response to muramyl dipeptide; response to organic substance; response to progesterone stimulus; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; viral reproduction

0.05毫克(大肠杆菌)

190美元

0.2毫克(大肠杆菌)

460

0.5毫克(大肠杆菌)

750

1毫克(大肠杆菌)

1180