ELISA/assay

大鼠甲平滑肌肌动蛋白酶联免疫吸附试剂盒

MBS720046

48-Strip-Wells-(Comp

470美元

酶联免疫吸附试剂盒

大鼠甲平滑肌肌动蛋白酶联免疫吸附试剂盒

甲平滑肌肌动蛋白

大鼠a平滑肌肌动蛋白酶联免疫吸附试剂盒

actin, aortic smooth muscle; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

甲SMA

a-SMA

大动脉平滑肌肌动蛋白alpha2;大动脉平滑肌肌动蛋白alpha2;AAT6;ACTSA;MYMY5;ACTSA;ACTVS;GIG46

ACTA_HUMAN

大鼠

This assay has high sensitivity and excellent specificity for detection of SMAalpha. No significant cross-reactivity or interference between SMAalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SMAalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0 ng/mL.

信号转导

Intended Uses This SMAalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SMAalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SMAalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SMAalpha. Standards or samples are then added to the microtiter plate wells and SMAalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SMAalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SMAalpha are added to each well to "sandwich” the SMAalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SMAalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMAalpha concentration in each sample is interpolated from this standard curve.

213688375

NP_001135417.1

NM_001141945.1

42,009 Da

Muscle Contraction Pathway (106261); Myometrial Relaxation And Contraction Pathways (198759); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (198903); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: protein kinase binding; ATP binding. Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction. Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6

48-Strip-Wells-(Competitive)

470美元

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675