产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Guinea pig Inducible nitric oxide synthase ELISA Kit
目录 :
MBS722517
规格 :
48-Strip-Wells
价格 :
470美元
更多信息或购买 :
产品信息
目录号 :
MBS722517
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Guinea pig Inducible nitric oxide synthase ELISA Kit
产品简称 :
Inducible nitric oxide synthase
其他名称 :
inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase
产品基因名称 :
iNOS
其他基因名称 :
Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; CG11143; MIP synthase; IPS; MI-1-P synthase
UniProt数据库进入名 :
INO1_DROME
反应物种 :
豚鼠
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
产品种类 :
神经生物学
产品描述 :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This BG INOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig INOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY INOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INOS antibody and an INOS-HRP conjugate. The assay sample and buffer are incubated together with INOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INOS concentration since INOSfrom samples and INOS-HRP conjugate compete for the anti-INOS antibody binding site. Since the number of sites is limited, as more sites are occupied by INOS from the sample, fewer sites are left to bind INOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INOS concentration in each sample is interpolated from this standard curve.
NCBI GI登录号 :
17137626
NCBI登录号 :
NP_477405.1
NCBI基因登录号 :
NM_058057.6
NCBI分子量 :
62,249 Da
NCBI信号通路 :
Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (1006205); Inositol Phosphate Metabolism Pathway (362); Metabolic Pathways (132021); Metabolism Pathway (1006204); Synthesis Of IP2, IP, And Ins In The Cytosol Pathway (1006210)
尺寸1 :
48-Strip-Wells
价格1 :
470美元
尺寸2 :
96-Strip-Wells
价格2 :
675
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。
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