产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
豚鼠白细胞介素13酶联免疫吸附试剂盒
目录 :
MBS724292
规格 :
48-Strip-Wells
价格 :
470美元
更多信息或购买 :
产品信息
目录号 :
MBS724292
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
豚鼠白细胞介素13酶联免疫吸附试剂盒
产品简称 :
白细胞介素13
其他名称 :
白细胞介素13;白细胞介素13;白细胞介素13;白细胞介素13
产品基因名称 :
IL-13
其他基因名称 :
白细胞介素-13;白细胞介素-13;P600;IL-13;NC30;IL-13
UniProt数据库进入名 :
IL13_HUMAN
反应物种 :
豚鼠
特异性 :
This assay has high sensitivity and excellent specificity for detection of IL-13. No significant cross-reactivity or interference between IL-13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-13 and all the analogues, therefore, cross reaction may still exist in some cases.
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
其他信息2 :
Intended Uses: This IL-13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IL-13. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
产品种类 :
免疫学
产品描述 :
Principle of the assay: IL-13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-13 antibody and an IL-13-HRP conjugate. The assay sample and buffer are incubated together with IL-13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-13 concentration since IL-13 from samples and IL-13-HRP conjugate compete for the anti-IL-13 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-13 from the sample, fewer sites are left to bind IL-13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-13 concentration in each sample is interpolated from this standard curve.
NCBI GI登录号 :
1045452
NCBI登录号 :
AAB01681.1
NCBI分子量 :
15,816 Da
NCBI信号通路 :
Asthma Pathway (83120); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493); Glucocorticoid Receptor Regulatory Network Pathway (138014); IL12 Signaling Mediated By STAT4 Pathway (137936); Inflammatory Bowel Disease (IBD) Pathway (842771)
NCBI总结 :
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane. Molecular Function: protein binding; interleukin-13 receptor binding; cytokine activity. Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; response to lipopolysaccharide; signal transduction; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; cell-cell signaling; positive regulation of B cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; cell motility; inflammatory response; positive regulation of macrophage activation. Disease: Asthma, Susceptibility To; Allergic Rhinitis
尺寸1 :
48-Strip-Wells
价格1 :
470美元
尺寸2 :
96-Strip-Wells
价格2 :
675
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。