产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
兔白细胞介素1beta酶联免疫吸附试剂盒
目录 :
MBS725024
规格 :
48-Strip-Wells
价格 :
470美元
更多信息或购买 :
文章摘录数: 1
参考文献
Tseng C, Chen Z, Renn T, Hsiao S, Burnouf T. Solvent/Detergent Virally Inactivated Serum Eye Drops Restore Healthy Ocular Epithelium in a Rabbit Model of Dry-Eye Syndrome. PLoS ONE. 2016;11:e0153573 pubmed 出版商
产品信息
目录号 :
MBS725024
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
兔白细胞介素1beta酶联免疫吸附试剂盒
产品简称 :
白细胞介素1beta
产品名称同义词 :
兔白细胞介素1b酶联免疫吸附试剂盒
其他名称 :
interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1, beta; Catabolin
产品基因名称 :
IL-1beta
产品基因名称同义词 :
IL-1b
其他基因名称 :
IL1B;IL1B;IL-1;IL1F2;IL1-乙;IL1F2;IL-1乙
UniProt数据库进入名 :
IL1B_HUMAN
反应物种 :
特异性 :
This assay has high sensitivity and excellent specificity for detection of IL-1beta. No significant cross-reactivity or interference between IL-1beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1beta and all the analogues, therefore, cross reaction may still exist in some cases.
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
产品种类 :
免疫学
产品描述 :
Intended Uses: This IL-1beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IL-1beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay IL-1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-1beta antibody and an IL-1beta-HRP conjugate. The assay sample and buffer are incubated together with IL-1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1beta concentration since IL-1beta from samples and IL-1beta-HRP conjugate compete for the anti-IL-1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1beta from the sample, fewer sites are left to bind IL-1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1beta concentration in each sample is interpolated from this standard curve.
产品引用 :
Tseng C-L, Chen Z-Y, Renn T-Y, Hsiao S-H, Burnouf T (2016) Solvent/Detergent Virally Inactivated Serum Eye Drops Restore Healthy Ocular Epithelium in a Rabbit Model of Dry-Eye Syndrome. PLoS ONE 11(4): e0153573. doi:10.1371/journal. pone.0153573
NCBI GI登录号 :
10835145
NCBI登录号 :
NP_000567.1
NCBI基因登录号 :
NM_000576.2
NCBI分子量 :
30,748 Da
NCBI信号通路 :
African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076)
NCBI总结 :
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of NF-kappaB import into nucleus; positive regulation of lipid catabolic process; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; cell-cell signaling; positive regulation of phagocytosis; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; smooth muscle adaptation; interleukin-1 beta production; positive regulation of interleukin-6 production; positive regulation of angiogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; sequestering of triacylglycerol; positive regulation of histone phosphorylation; apoptosis; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein export from nucleus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; response to ATP; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion; embryo implantation. Disease: Gastric Cancer, Hereditary Diffuse
尺寸1 :
48-Strip-Wells
价格1 :
470美元
尺寸2 :
96-Strip-Wells
价格2 :
675
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。