产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
小鼠胰岛素降解酶酶联免疫吸附试剂盒
目录 :
MBS725082
规格 :
48-Strip-Wells
价格 :
470美元
更多信息或购买 :
文章摘录数: 2
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产品信息
目录号 :
MBS725082
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
小鼠胰岛素降解酶酶联免疫吸附试剂盒
产品简称 :
胰岛素降解酶
其他名称 :
胰岛素降解酶;胰岛素降解酶
产品基因名称 :
IDE
其他基因名称 :
ide
反应物种 :
小鼠
特异性 :
This assay has high sensitivity and excellent specificity for detection of IDE. No significant cross-reactivity or interference between IDE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IDE and all the analogues, therefore, cross reaction may still exist in some cases.
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
其他信息2 :
Intended Uses: This IDE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IDE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
产品种类 :
免疫学
产品描述 :
Principle of the assay: IDE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IDE antibody and an IDE-HRP conjugate. The assay sample and buffer are incubated together with IDE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDE concentration since IDE from samples and IDE-HRP conjugate compete for the anti-IDE antibody binding site. Since the number of sites is limited, as more sites are occupied by IDE from the sample, fewer sites are left to bind IDE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDE concentration in each sample is interpolated from this standard curve.
NCBI GI登录号 :
350535204
NCBI登录号 :
NP_001233926.1
NCBI基因登录号 :
NM_001246997.1
尺寸1 :
48-Strip-Wells
价格1 :
470美元
尺寸2 :
96-Strip-Wells
价格2 :
675
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。