ELISA/assay

小鼠血小板衍生生长因子D酶联免疫吸附试剂盒

MBS725417

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

小鼠血小板衍生生长因子D酶联免疫吸附试剂盒

血小板衍生生长因子D

platelet-derived growth factor D isoform 2; Platelet-derived growth factor D; platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B; platelet derived growth factor D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B

PDGFD

PDGFD; PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B; IEGF; SCDGFB; MSTP036; UNQ1899/PRO4345; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form

PDGFD_HUMAN

小鼠

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This PDGF-D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PDGF-D. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY PDGF-D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDGF-D antibody and an PDGF-D-HRP conjugate. The assay sample and buffer are incubated together with PDGF-D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGF-D concentration since PDGF-D from samples and PDGF-D-HRP conjugate compete for the anti-PDGF-D antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGF-D from the sample, fewer sites are left to bind PDGF-D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGF-D concentration in each sample is interpolated from this standard curve.

15451921

NP_149126.1

NM_033135.3

42,167 Da

Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Gap Junction Pathway (83072); Gap Junction Pathway (483); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Prostate Cancer Pathway (83111)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Oncoprotein. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth factor activity; platelet-derived growth factor receptor binding. Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation

48-Strip-Wells

470美元

96-Strip-Wells

675