ELISA/assay

Human Oxidized lowdensity lipoprotein ELISA Kit

MBS727303

48-Strip-Wells-(Competitive)

440美元

酶联免疫吸附试剂盒

Human Oxidized lowdensity lipoprotein ELISA Kit

[Oxidized lowdensity lipoprotein]

[OxLDL]

人类

This assay has high sensitivity and excellent specificity for detection of oxLDL. No significant cross-reactivity or interference between oxLDL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between oxLDL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Detection Range: 2.5-50ng/mL. Sensitivity: 1.0ng/mL

Cardiovascular

Intended Uses: This oxLDL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human oxLDL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay OxLDL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for oxLDL. Standards or samples are then added to the microtiter plate wells and oxLDL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of oxLDL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for oxLDL are added to each well to "sandwich" the oxLDL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain oxLDL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The oxLDL concentration in each sample is interpolated from this standard curve.

48-Strip-Wells-(Competitive)

440美元

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415