产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
大鼠表皮生长因子受体2细胞外区域酶联免疫吸附试剂盒
目录 :
MBS728852
规格 :
48-Strip-Wells-(Comp
价格 :
470美元
更多信息或购买 :
产品信息
目录号 :
MBS728852
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
大鼠表皮生长因子受体2细胞外区域酶联免疫吸附试剂盒
产品简称 :
表皮生长因子受体2细胞外区域
其他名称 :
receptor tyrosine-protein kinase erbB-2 isoform a; Receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
产品基因名称 :
HER-2 ECD
其他基因名称 :
ERBB2; ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; HER2; MLN19; NEU; NGL; MLN 19
UniProt数据库进入名 :
ERBB2_HUMAN
反应物种 :
大鼠
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
其他信息2 :
Intended Uses: This HER-2/nue ECD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HER-2/nue ECD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
产品描述 :
Principle of the Assay: HER-2/nue ECD ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HER-2/nue ECD. Standards or samples are then added to the microtiter plate wells and HER-2/nue ECD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HER-2/nue ECD present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for HER-2/nue ECD are added to each well to "sandwich" the HER-2/nue ECD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HER-2/nue ECD and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HER-2/nue ECD concentration in each sample is interpolated from this standard curve.
NCBI GI登录号 :
54792096
NCBI登录号 :
NP_004439.2
NCBI基因登录号 :
NM_004448.3
NCBI分子量 :
97,253 Da
NCBI信号通路 :
Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535)
NCBI总结 :
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033. Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; Kinase, protein; Membrane protein, integral; TK group; EGFR family. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex. Molecular Function: protein dimerization activity; protein C-terminus binding; identical protein binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; ATP binding. Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion; positive regulation of translation; nerve growth factor receptor signaling pathway; wound healing; heart development; protein amino acid autophosphorylation; motor axon guidance; signal transduction; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; negative regulation of immature T cell proliferation in the thymus; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; regulation of angiogenesis; positive regulation of cell growth; peripheral nervous system development; cell proliferation; innate immune response; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
尺寸1 :
48-Strip-Wells-(Competitive)
价格1 :
470美元
尺寸2 :
48-Strip-Wells-(Sandwich)
价格2 :
470
size3 :
96-Strip-Wells-(Competitive)
价格3 :
675
size4 :
96-Strip-Wells-(Sandwich)
price4 :
675
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。