ELISA/assay

大鼠alpha2抗纤溶酶酶联免疫吸附试剂盒

MBS729223

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

大鼠alpha2抗纤溶酶酶联免疫吸附试剂盒

alpha2抗纤溶酶

大鼠alpha2抗纤溶酶酶联免疫吸附试剂盒;alpha2抗纤溶酶;a2抗纤溶酶;2抗纤溶酶;alpha2抗纤溶酶(大鼠)

alpha-2-antiplasmin; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; alpha-2-PI serine protease inhibitor

alpha2-AP

a2-AP;2-AP

SERPINF2;丝氨酸蛋白酶抑制剂

大鼠

Sensitivity: The sensitivity in this assay is 0.1 ng/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of A2-AP. No significant cross-reactivity or interference between A2-AP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This APalpha2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat APalpha2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

大鼠酶联免疫吸附试剂盒

Principle of the Assay: APalpha2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APalpha2 antibody and an APalpha2-HRP conjugate. The assay sample and buffer are incubated together with APalpha2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APalpha2 concentration since APalpha2 from samples and APalpha2-HRP conjugate compete for the anti-APalpha2 antibody binding site. Since the number of sites is limited, as more sites are occupied by APalpha2 from the sample, fewer sites are left to bind APalpha2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APalpha2 concentration in each sample is interpolated from this standard curve.

130488651

NP_001076210.1

NM_001082741.2

48-Strip-Wells

470美元

96-Strip-Wells

675