ELISA/assay

小鼠溴脱氧尿苷酶联免疫吸附试剂盒

MBS731098

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

小鼠溴脱氧尿苷酶联免疫吸附试剂盒

溴脱氧尿苷

溴化去氧尿苷

小鼠

This assay has high sensitivity and excellent specificity for detection of BrdU. No significant cross-reactivity or interference between BrdU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BrdU and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pmol/ml.

Intended Uses: This BrdU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BrdU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: BrdU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BrdU antibody and an BrdU-HRP conjugate. The assay sample and buffer are incubated together with BrdU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BrdU concentration since BrdU from samples and BrdU-HRP conjugate compete for the anti-BrdU antibody binding site. Since the number of sites is limited, as more sites are occupied by BrdU from the sample, fewer sites are left to bind BrdU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BrdU concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470美元

96-Strip-Wells

675