ELISA/assay

Human Carbonic Anhydarse XII (CA12) ELISA Kit

MBS733811

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

Human Carbonic Anhydarse XII (CA12) ELISA Kit

Human Carbonic Anhydarse XII (CA12)

CA12

人类

This assay has high sensitivity and excellent specificity for detection of CA12. No significant cross-reactivity or interference between CA12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CA12 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This CA12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CA12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

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Principle of the Assay: CA12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CA12. Standards or samples are then added to the microtiter plate wells and CA12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CA12 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CA12 are added to each well to "sandwich" the CA12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CA12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA12 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470美元

96-Strip-Wells

675