ELISA/assay

人类C4结合蛋白, 甲酶联免疫吸附试剂盒

MBS733812

48-Strip-Wells-(Comp

470美元

酶联免疫吸附试剂盒

人类C4结合蛋白, 甲酶联免疫吸附试剂盒

C4结合蛋白, 甲

C4b-binding protein alpha chain; C4b-binding protein alpha chain; C4b-binding protein alpha chain; proline-rich protein; complement component 4 binding protein, alpha; Proline-rich protein; PRP

C4BPa

C4BPA;C4BPA;PRP;C4BP;C4BP;C4bp;PRP

C4BPA_HUMAN

人类

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

免疫学

Intended Uses: This C4BPa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C4BPa. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: C4BPa ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C4BPa. Standards or samples are then added to the microtiter plate wells and C4BPa if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C4BPa present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C4BPa are added to each well to "sandwich" the C4BPa immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C4BPa and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4BPa concentration in each sample is interpolated from this standard curve.

4502503

NP_000706.1

NM_000715.3

67,033 Da

CD40/CD40L Signaling Pathway (138061); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Pertussis Pathway (218111); Pertussis Pathway (218099); Regulation Of Complement Cascade Pathway (576254)

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; extracellular region; plasma membrane. Molecular Function: protein binding. Biological Process: negative regulation of complement activation, classical pathway; regulation of complement activation; positive regulation of protein catabolic process; innate immune response; complement activation, classical pathway

48-Strip-Wells-(Competitive)

470美元

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675