ELISA/assay

猪a1酸糖蛋白酶联免疫吸附试剂盒

MBS739684

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

猪a1酸糖蛋白酶联免疫吸附试剂盒

a1酸糖蛋白

alpha-1-acid glycoprotein-2, partial; Alpha-1-acid glycoprotein-2; Alpha-1-acid glycoprotein-2

A1-AGP

Q60839_MOUSE

猪

This assay has high sensitivity and excellent specificity for detection of alpha1AGP. No significant cross-reactivity or interference between alpha1AGP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha1AGP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1mg/mL

免疫学

Intended Uses: This alpha1AGP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine alpha1AGP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: alpha1AGP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for alpha1AGP. Standards or samples are then added to the microtiter plate wells and alpha1AGP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alpha1AGP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alpha1AGP are added to each well to "sandwich" the alpha1AGP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alpha1AGP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1AGP concentration in each sample is interpolated from this standard curve.

1209502

AAA91124.1

2,843 Da

48-Strip-Wells

470美元

96-Strip-Wells

675