ELISA/assay

牛血小板衍生生长因子D酶联免疫吸附试剂盒

MBS742718

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

牛血小板衍生生长因子D酶联免疫吸附试剂盒

血小板衍生生长因子D

platelet-derived growth factor D isoform 2; Platelet-derived growth factor D; platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B; platelet derived growth factor D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B

PDGFD

PDGFD; PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B; IEGF; SCDGFB; MSTP036; UNQ1899/PRO4345; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form

PDGFD_HUMAN

牛

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This PDGF-D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PDGF-D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: PDGF-D ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDGF-D. Standards or samples are then added to the microtiter plate wells and PDGF-D if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDGF-D present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PDGF-D are added to each well to "sandwich" the PDGF-D immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDGF-D and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGF-D concentration in each sample is interpolated from this standard curve.

15451921

NP_149126.1

NM_033135.3

42,167 Da

Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Gap Junction Pathway (83072); Gap Junction Pathway (483); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Prostate Cancer Pathway (83111)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Oncoprotein; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth factor activity; platelet-derived growth factor receptor binding. Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation

48-Strip-Wells

470美元

96-Strip-Wells

675