ELISA/assay

Monkey Mast Cell Chymase ELISA Kit

MBS746927

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

Monkey Mast Cell Chymase ELISA Kit

Mast Cell Chymase

mast cell chymase; Chymase; chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I

MCC

CMA1; CMA1; CYH; MCT1; chymase; CYH; CYM

CMA1_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of CMA. No significant cross-reactivity or interference between CMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CMA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CMA antibody and an CMA-HRP conjugate. The assay sample and buffer are incubated together with CMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CMA concentration since CMA from samples and CMA-HRP conjugate compete for the anti-CMA antibody binding site. Since the number of sites is limited, as more sites are occupied by CMA from the sample, fewer sites are left to bind CMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CMA concentration in each sample is interpolated from this standard curve.

180542

AAA52020.1

27,325 Da

ACE Inhibitor Pathway (198763); Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (477114)

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Protease; EC 3.4.21.39; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding. Biological Process: interleukin-1 beta biosynthetic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of angiogenesis; cellular protein metabolic process; regulation of inflammatory response; midbrain development; protein processing; angiotensin maturation; proteolysis

48-Strip-Wells

470美元

96-Strip-Wells

675