ELISA/assay

Mouse Creatine phosphokinase ELISA Kit

MBS755902

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

Mouse Creatine phosphokinase ELISA Kit

Creatine phosphokinase

肌酸激酶 M类型;肌酸激酶 M类型;肌酸激酶 M类型;M-CK;肌肉肌酸激酶 ;肌酸激酶 M链;肌酸激酶 M链;M-CK

CPK

CKM;CKM

KCRM_RABIT

小鼠

This assay has high sensitivity and excellent specificity for detection of CPK. No significant cross-reactivity or interference between CPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CPK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.

126723370

NP_001075708.1

NM_001082239.1

43,112 Da

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Kinase, other; Amino Acid Metabolism - arginine and proline; EC 2.7.3.2. Cellular Component: cytoplasm. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; phosphorylation

48-Strip-Wells

470美元

96-Strip-Wells

675