ELISA/assay

人类1, 3乙(BDG)酶联免疫吸附试剂盒

MBS756415

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

人类1, 3乙(BDG)酶联免疫吸附试剂盒

1, 3乙(BDG)

BDG

人类

This assay has high sensitivity and excellent specificity for detection of BDG. No significant cross-reactivity or interference between BDG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BDG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses This BDG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BDG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BDG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BDG antibody and an BDG-HRP conjugate. The assay sample and buffer are incubated together with BDG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDG concentration since BDG from samples and BDG-HRP conjugate compete for the anti-BDG antibody binding site. Since the number of sites is limited, as more sites are occupied by BDG from the sample, fewer sites are left to bind BDG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDG concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470美元

96-Strip-Wells

675