产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
人类甲状旁腺激素相关蛋白酶联免疫吸附试剂盒
目录 :
MBS760563
规格 :
48-Strip-Wells
价格 :
300美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS760563 图像 1
产品信息
目录号 :
MBS760563
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
人类甲状旁腺激素相关蛋白酶联免疫吸附试剂盒
产品简称 :
[甲状旁腺激素相关蛋白]
产品名称同义词 :
[PTHrP (Parathyroid Hormone Related Protein)/PTHLH/BDE2/HHM/Osteostatin/PTHRP/parathyroid hormone-like hormone/Parathyroid hormone-like protein/parathyroid hormone-related protein/PLPosteostatin/PTHR/PTH-related protein/PTH-rP/parathyroid hormone-like related protein]
其他名称 :
[parathyroid hormone-related protein; Parathyroid hormone-related protein; parathyroid hormone-related protein; parathyroid hormone-like hormone; Parathyroid hormone-like protein; PLP]
产品基因名称 :
[PTHLH]
其他基因名称 :
[PTHLH; PTHLH; HHM; PLP; BDE2; PTHR; PTHRP; PTHRP; PTH-rP; PTHrP; PLP]
UniProt数据库进入名 :
PTHR_HUMAN
反应物种 :
人类
序列长度 :
177
特异性 :
This assay has high sensitivity and excellent specificity for detection of PTHLH. No significant cross-reactivity or interference between PTHLH and analogues was observed.
储存稳定性 :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 15.6-1000pg/ml. Sensitivity: 9.375pg/ml
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PTHLH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PTHLH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
产品描述 :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PTHLH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PTHLH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PTHLH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PTHLH can be calculated.
NCBI GI登录号 :
190726
NCBI登录号 :
AAA60221.1
NCBI分子量 :
23,942 Da
NCBI信号通路 :
Class B/2 (Secretin Family Receptors) Pathway (1269570); Endochondral Ossification Pathway (198812); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543); Signaling Events Mediated By The Hedgehog Family Pathway (137950)
NCBI总结 :
The protein encoded by this gene is a member of the parathyroid hormone family. This hormone, via its receptor, PTHR1, regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. It is responsible for most cases of humoral hypercalcemia of malignancy, and mutations in this gene are associated with brachydactyly type E2 (BDE2). Alternatively spliced transcript variants have been found for this gene. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, downstream of the initiator AUG codon, resulting in nuclear forms of this hormone. [provided by RefSeq, Nov 2013]
UniProt数据库总结 :
PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p12.1-p11.2. Cellular Component: cytoplasm; extracellular region; extracellular space; Golgi apparatus; nucleoplasm. Molecular Function: hormone activity; peptide hormone receptor binding. Biological Process: alveolus development; cAMP metabolic process; cell-cell signaling; endochondral ossification; endoderm development; epidermis development; epithelial cell differentiation; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; negative regulation of transcription factor activity; osteoblast development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of gene expression; skeletal development; surfactant homeostasis. Disease: Brachydactyly, Type E2
尺寸1 :
48-Strip-Wells
价格1 :
300美元
尺寸2 :
96-Strip-Wells
价格2 :
415
size3 :
5x96-Strip-Wells
价格3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。