产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
猪GAL3(半乳糖凝集素3)酶联免疫吸附试剂盒
目录 :
MBS7606136
规格 :
48-Strip-Wells
价格 :
300美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS7606136 图像 1
产品信息
目录号 :
MBS7606136
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
猪GAL3(半乳糖凝集素3)酶联免疫吸附试剂盒
产品简称 :
[GAL3(半乳糖凝集素3)]
其他名称 :
[galectin-3 isoform 1; Galectin-3; galectin-3; galectin 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen]
产品基因名称 :
[GAL3]
其他基因名称 :
[LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP]
反应物种 :
序列长度 :
250
特异性 :
This assay has high sensitivity and excellent specificity for detection of GAL3. No significant cross-reactivity or interference between GAL3 and analogues was observed.
储存稳定性 :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 3.12-200pg/ml. Sensitivity: <1.875pg/ml
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GAL3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GAL3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
产品描述 :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GAL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GAL3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GAL3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GAL3 can be calculated.
NCBI GI登录号 :
115430223
NCBI登录号 :
NP_002297.2
NCBI基因登录号 :
NM_002306.3
UniProt数据库登录号 :
P17931
NCBI分子量 :
26,152 Da
NCBI信号通路 :
AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)
NCBI总结 :
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
UniProt数据库总结 :
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
尺寸1 :
48-Strip-Wells
价格1 :
300美元
尺寸2 :
96-Strip-Wells
价格2 :
415
size3 :
5x96-Strip-Wells
价格3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。