ELISA/assay

Bovine Lep (Leptin) ELISA Kit

MBS7606202

48-Strip-Wells

300美元

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Bovine Lep (Leptin) ELISA Kit

[Lep (Leptin)]

[Lep]

牛

This assay has high sensitivity and excellent specificity for detection of Lep. No significant crossreactivity or interference between Lep and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lep were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lep were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Lep antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Lep antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lep amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lep can be calculated.

4557715

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48-Strip-Wells

300美元

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425