产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
兔白细胞介素8酶联免疫吸附试剂盒
目录 :
MBS763266
规格 :
48-Strip-Wells
价格 :
300美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS763266 图像 1
产品信息
目录号 :
MBS763266
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
兔白细胞介素8酶联免疫吸附试剂盒
产品简称 :
[白细胞介素8]
其他名称 :
[interleukin-8; Interleukin-8; interleukin-8; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1]
产品基因名称 :
[IL-8]
其他基因名称 :
[CXCL8;CXCL8;白细胞介素-8;NAF;GCP1;LECT;LUCT;NAP1;GCP-1;LYNAP;MDNCF;MONAP;NAP-1;白细胞介素-8;IL-8;GCP-1;MDNCF;MONAP;NAP-1;LYNAP;NAF]
UniProt数据库进入名 :
IL8_HUMAN
反应物种 :
序列长度 :
99
特异性 :
This assay has high sensitivity and excellent specificity for detection ofIL-6. No significant cross-reactivity or interference betweenIL-6 and analogues was observed.
储存稳定性 :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-6 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
产品描述 :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated.
NCBI GI登录号 :
10834978
NCBI登录号 :
NP_000575.1
NCBI基因登录号 :
NM_000584.3
NCBI分子量 :
11,098 Da
NCBI信号通路 :
AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); ATF-2 Transcription Factor Network Pathway (138006); ATF4 Activates Genes Pathway (1268762); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cellular Senescence Pathway (1270426)
NCBI总结 :
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 4q13-q21. Cellular Component: extracellular region; extracellular space; intracellular. Molecular Function: chemokine activity; interleukin-8 receptor binding; protein binding. Biological Process: angiogenesis; calcium-mediated signaling; cell cycle arrest; cell motility; cellular protein metabolic process; chemotaxis; embryonic gut development; G-protein coupled receptor protein signaling pathway; immune response; induction of positive chemotaxis; inflammatory response; negative regulation of cell proliferation; negative regulation of G-protein coupled receptor protein signaling pathway; neutrophil activation; neutrophil chemotaxis; positive regulation of angiogenesis; receptor internalization; regulation of cell adhesion; regulation of retroviral genome replication; response to molecule of bacterial origin; signal transduction; unfolded protein response
尺寸1 :
48-Strip-Wells
价格1 :
300美元
尺寸2 :
96-Strip-Wells
价格2 :
415
size3 :
5x96-Strip-Wells
价格3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。