ELISA/assay

Human Proline-rich acidic protein 1 ELISA Kit

MBS765465

48-Strip-Wells

300美元

酶联免疫吸附试剂盒

Human Proline-rich acidic protein 1 ELISA Kit

[Proline-rich acidic protein 1]

[PRAP1 (Proline-rich acidic protein 1)/Uterine-specific proline-rich acidic protein/Uterine-specific proline-rich acidic protein]

[proline-rich acidic protein 1 isoform 1; Proline-rich acidic protein 1; proline-rich acidic protein 1; proline-rich acidic protein 1; Epididymis tissue protein Li 178; Uterine-specific proline-rich acidic protein]

[PRAP1]

[PRAP1;PRAP1;UPA;PRO1195;UPA]

PRAP1_HUMAN

人类

151

This assay has high sensitivity and excellent specificity for detection of PPP3CC. No significant cross-reactivity or interference between PPP3CC and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PPP3CC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PPP3CC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PPP3CC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PPP3CC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PPP3CC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PPP3CC can be calculated.

223633959

NP_660203.3

NM_145202.4

16,332 Da

PRAP1: May play an important role in maintaining normal growth homeostasis in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q26.3. Cellular Component: extracellular region. Molecular Function: protein binding

48-Strip-Wells

300美元

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425