ELISA/assay

Monkey Apolipoprotein B100 ELISA Kit

MBS765632

48-Strip-Wells

300美元

酶联免疫吸附试剂盒

Monkey Apolipoprotein B100 ELISA Kit

[载脂蛋白B100]

[载脂蛋白b-100;载脂蛋白b-100;载脂蛋白b-100;载脂蛋白b]

[ApoB100]

[载脂蛋白B;载脂蛋白B;FLDB;LDLCQ4;载脂蛋白B 48;载脂蛋白B 100;载脂蛋白B-100;载脂蛋白B-48]

APOB_HUMAN

Monkey

4563

This assay has high sensitivity and excellent specificity for detection of ApoB100. No significant cross-reactivity or interference between ApoB100 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 28.12-1800ng/ml. Sensitivity: < 16ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ApoB100 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ApoB100 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ApoB100 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ApoB100 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ApoB100 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ApoB100 can be calculated.

105990532

NP_000375.2

NM_000384.2

515,605 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1269897); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Chylomicron-mediated Lipid Transport Pathway (1270006); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (1269340); LDL-mediated Lipid Transport Pathway (1270008); Lipid Digestion, Mobilization, And Transport Pathway (1270002); Lipoprotein Metabolism Pathway (1270005)

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted; Carrier; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p24-p23. Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; cytosol; early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane. Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding. Biological Process: artery morphogenesis; blood coagulation; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fat-soluble vitamin metabolic process; fertilization; in utero embryonic development; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; phototransduction, visible light; post-embryonic development; receptor-mediated endocytosis; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to lipopolysaccharide; response to selenium ion; response to virus; retinoid metabolic process; sperm motility; spermatogenesis; triacylglycerol catabolic process; triacylglycerol mobilization; vitamin metabolic process. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1

48-Strip-Wells

300美元

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425