抗体

小鼠抗大鼠GluN2B/NR2B单克隆IgG2b

MBS800075

0.1毫克

390美元

单克隆

大鼠

未共轭

S59-36

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

抗体

小鼠抗大鼠GluN2B/NR2B单克隆IgG2b

NR2B

NMDAR2B; hNR3; MGC142180; glutamate receptor subunit epsilon-2; NR3

glutamate receptor ionotropic, NMDA 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B

Grin2b;Grin2b;GluN2B;GluN2B;NMDAR2B;NR2B

NMDE2_RAT

单克隆

IgG2b

S59-36

大鼠

人类, 大鼠, 小鼠

1482

Detects ~166kDa. No cross-reactivity against NR2A

蛋白质G纯化

1毫克/毫升

-20 degree C. Avoid freeze/thaw cycles. Shipping Temperature: 4 degree C

Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: (1:1000), IHC: (1:1000), ICC/IF (1:1000). Optimal dilutions for assays should be determined by the user.

Certificate of Analysis: 1ug/mL was sufficient for detection of NR2B in 10ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Immunogen: Fusion protein amino acids 20-127 (extracellular N-terminus) of rat NR2B.

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. Cellular Localization: Cell membrane, Cell Junction, synapse, postsynaptic cell membrane. Research Areas: Neuroscience, Ion Channels, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Neurodegeneration. Conjugates: Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

离子通道, 神经科学

Background Info: Detects ~166kDa. No cross-reactivity against NR2A. Scientific Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (3). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (4).

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zhuo M. (2009) Mol Brain. 2(1): 4 4. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.

6980984

NP_036706.1

NM_012574.1

Q00960

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Circadian Entrainment Pathway (698787); Circadian Entrainment Pathway (699872)

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Protein type: Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated; Membrane protein, integral. Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; Z disc; N-methyl-D-aspartate selective glutamate receptor complex. Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cell adhesion molecule binding; cation channel activity; receptor binding; D2 dopamine receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; behavioral response to pain; response to magnesium ion; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; associative learning; cation transport; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; sensory organ development; regulation of postsynaptic membrane potential; response to ethanol; suckling behavior; long-term memory; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to cytokine stimulus; regulation of MAPKKK cascade; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; response to amine stimulus

0.1毫克

390美元