产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗水闸蛋白5抗体
目录 :
MBS821163
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
unidentified
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 牛
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Claudin 5 expression in HeLa (A), mouse liver (B), rat lung (C) whole cell lysates.
图像 2 :
Immunohistochemical analysis of Claudin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
图像 3 :
Immunofluorescent analysis of Claudin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS821163
产品类型 :
抗体
产品全称 :
抗水闸蛋白5抗体
产品简称 :
[水闸蛋白5]
产品名称同义词 :
[AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF]
其他名称 :
[claudin-5; Claudin-5; claudin-5; TMDVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome; claudin 5; Transmembrane protein deleted in VCFS; TMDVCF]
产品基因名称 :
[CLDN5]
其他基因名称 :
[CLDN5;CLDN5;AWAL;BEC1;TMVCF;CPETRL1;AWAL;TMVCF;TMDVCF]
UniProt数据库进入名 :
CLD5_HUMAN
克隆性 :
多克隆
宿主 :
未知
反应物种 :
人类, 小鼠, 大鼠, 牛, 猪, 兔
序列长度 :
303
特异性 :
Recognizes endogenous levels of Claudin 5 protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Ships on blue ice. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学)
应用笔记 :
免疫印迹(1/500 - 1/1000);IH(1/100 - 1/200);IF/免疫细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其他信息2 :
Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 5. The exact sequence is proprietary.
产品描述 :
兔多克隆抗体水闸蛋白5
NCBI GI登录号 :
195947374
NCBI登录号 :
NP_001124333.1
NCBI基因登录号 :
NM_001130861.1
UniProt数据库登录号 :
O00501
NCBI信号通路 :
Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)
NCBI总结 :
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt数据库总结 :
Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Protein type: Membrane protein, integral; Cytoskeletal; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: tight junction; plasma membrane; integral to membrane; intercellular junction. Molecular Function: identical protein binding; structural molecule activity. Biological Process: calcium-independent cell-cell adhesion
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。