产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗PDGFR甲抗体
目录 :
MBS821212
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 狗, 鸡, 牛
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of PDGFR alpha expression in HepG2 (A), NIH3T3 (B), Saos2 (C), HeLa (D), A431 (E) whole cell lysates.
图像 2 :
图像 3 :
Immunofluorescent analysis of PDGFR alpha staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS821212
产品类型 :
抗体
产品全称 :
抗PDGFR甲抗体
产品简称 :
[PDGFR甲]
产品名称同义词 :
[PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140a]
其他名称 :
[platelet-derived growth factor receptor alpha; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor alpha; PDGF-R-alpha; CD140a antigen; PDGFRA/BCR fusion; CD140 antigen-like family member A; platelet-derived growth factor receptor 2; alpha-type platelet-derived growth factor receptor; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; platelet-derived growth factor receptor, alpha polypeptide; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD_antigen: CD140a]
产品基因名称 :
[血小板源性生长因子受体]
其他基因名称 :
[血小板源性生长因子受体;血小板源性生长因子受体;CD140A;血小板衍生的生长因子受体2;PDGFR-2;RHEPDGFRA;血小板衍生的生长因子受体2;RHEPDGFRA;PDGF-R;PDGFR甲;PDGFR-2]
UniProt数据库进入名 :
PGFRA_HUMAN
克隆性 :
多克隆
宿主 :
兔
反应物种 :
Human, Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig
序列长度 :
1089
特异性 :
Recognizes endogenous levels of PDGFR alpha protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
免疫印迹, IF/细胞化学
应用笔记 :
免疫印迹(1/500 - 1/1000)。 IF/细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Entrez Gene: 5156 (Human); 18595 (Mouse); 25267 (Rat). SwissProt: P16234 (Human); P26618 (Mouse); P20786 (Rat)
其他信息2 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PDGFR alpha. The exact sequence is proprietary.
产品描述 :
兔多克隆抗体PDGFR甲
NCBI GI登录号 :
5453870
NCBI登录号 :
NP_006197.1
NCBI基因登录号 :
NM_006206.4
UniProt数据库登录号 :
P16234
NCBI信号通路 :
ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549)
NCBI总结 :
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]
UniProt数据库总结 :
PDGFRA: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation. Particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Chromosomal rearrangments activate PDGFRalpha by fusion to BCR, causing atypical chronic myelogenous leukemia (CML), and to FIP1L1, causing idiopathic hypereosinophilic syndrome. Activating point mutations cause a minority of gastrointestinal stromal tumors (GIST). Promoter polymorphisms linked to neural tube defects including spina bifida, verified by mouse mutant model. Inhibitors: Gleevec, Sutent. OMIM: Two alternatively-spliced isoforms have been described. Protein type: Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Kinase, protein; TK group; PDGFR family. Chromosomal Location of Human Ortholog: 4q12. Cellular Component: microvillus; membrane; integral to plasma membrane; cytoplasm; plasma membrane; nucleus; intrinsic to plasma membrane; external side of plasma membrane. Molecular Function: vascular endothelial growth factor receptor activity; protein binding; protein homodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; platelet-derived growth factor alpha-receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: estrogen metabolic process; extracellular matrix organization and biogenesis; regulation of chemotaxis; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; wound healing; viral reproduction; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; palate development; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; Leydig cell differentiation; embryonic digestive tract morphogenesis; luteinization; positive regulation of cell proliferation; male genitalia development; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; adrenal gland development; in utero embryonic development; positive regulation of phosphoinositide 3-kinase activity; embryonic cranial skeleton morphogenesis; odontogenesis of dentine-containing teeth; embryonic skeletal morphogenesis; positive regulation of phosphoinositide 3-kinase cascade; cell activation; innate immune response; hemopoietic progenitor cell differentiation; positive regulation of DNA replication; lung development; positive regulation of cell migration. Disease: Gastrointestinal Stromal Tumor; Hypereosinophilic Syndrome, Idiopathic
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。