产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗半胱天冬酶1 p10抗体
目录 :
MBS821314
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
unidentified
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Caspase 1 p10 expression in HeLa (A), mouse spleen (B), rat spleen (C) whole cell lysates.
图像 2 :
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS821314
产品类型 :
抗体
产品全称 :
抗半胱天冬酶1 p10抗体
产品简称 :
[半胱天冬酶1 p10]
产品名称同义词 :
[IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45]
其他名称 :
[caspase-1 isoform beta; Caspase-1; caspase-1; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10]
产品基因名称 :
[胱天蛋白酶凋亡相关半胱氨酸肽酶1]
其他基因名称 :
[CASP1; CASP1; ICE; P45; IL1BC; IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme]
UniProt数据库进入名 :
CASP1_HUMAN
克隆性 :
多克隆
宿主 :
未知
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
383
特异性 :
Recognizes endogenous levels of Caspase 1 p10 protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
应用笔记 :
免疫印迹(1/500 - 1/1000), IF/免疫细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫荧光(IF)
其它信息1 :
Source: Rabbit
其他信息2 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1. The exact sequence is proprietary.
产品描述 :
兔多克隆抗体半胱天冬酶1 p10
NCBI GI登录号 :
4502573
NCBI登录号 :
NP_001214.1
NCBI基因登录号 :
NM_001223.4
UniProt数据库登录号 :
P29466
NCBI分子量 :
10,417 Da
NCBI信号通路 :
Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Caspase Cascade In Apoptosis Pathway (137974); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939)
NCBI总结 :
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
UniProt数据库总结 :
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.22.36; Apoptosis. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: cytoplasm; extracellular region; cytosol. Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; signal transduction; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。