产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗胶原4甲5抗体
目录 :
MBS8218169
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Collagen 4 alpha 5 expression in HeLa (A), mouse brain (B) whole cell lysates.
图像 2 :
Immunohistochemical analysis of Collagen 4 alpha 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
图像 3 :
Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS8218169
产品类型 :
抗体
产品全称 :
抗胶原4甲5抗体
产品简称 :
[胶原4甲5]
产品名称同义词 :
[胶原甲5(IV)链]
其他名称 :
[collagen alpha-5(IV) chain isoform 1; Collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; collagen, type IV, alpha 5]
产品基因名称 :
[COL4A5]
其他基因名称 :
[COL4A5;COL4A5;ATS;ASLN;CA54]
UniProt数据库进入名 :
CO4A5_HUMAN
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠
序列长度 :
1685
特异性 :
Recognizes endogenous levels of Collagen 4 alpha 5 protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
免疫印迹, IH, IF/细胞化学
应用笔记 :
免疫印迹(1/500 - 1/1000)。 免疫组化(1/100 - 1/200)。 IF/细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 4 alpha 5. The exact sequence is proprietary.
产品描述 :
兔多克隆抗体胶原4甲5
NCBI GI登录号 :
4502955
NCBI登录号 :
NP_000486.1
NCBI基因登录号 :
NM_000495.4
UniProt数据库登录号 :
P29400
NCBI信号通路 :
Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1270247); Axon Guidance Pathway (1270303); Collagen Biosynthesis And Modifying Enzymes Pathway (1270246); Collagen Formation Pathway (1270245); Developmental Biology Pathway (1270302); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1270244)
NCBI总结 :
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt数据库总结 :
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction. Molecular Function: extracellular matrix structural constituent. Biological Process: collagen catabolic process; extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development. Disease: Alport Syndrome, X-linked
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。