产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-Paxillin Antibody
目录 :
MBS8218302
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
unidentified
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS8218302 图像 1
Western blot analysis of Paxillin expression in HeLa (A) whole cell lysates.
图像 2 :
MyBioSource MBS8218302 图像 2
Immunohistochemical analysis of Paxillin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
图像 3 :
MyBioSource MBS8218302 图像 3
Immunofluorescent analysis of Paxillin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS8218302
产品类型 :
抗体
产品全称 :
Anti-Paxillin Antibody
产品简称 :
[Paxillin]
产品名称同义词 :
[Paxillin]
其他名称 :
[paxillin isoform 2; Paxillin; paxillin; paxillin]
产品基因名称 :
[PXN]
其他基因名称 :
[PXN;PXN]
UniProt数据库进入名 :
PAXI_HUMAN
克隆性 :
多克隆
宿主 :
未知
反应物种 :
人类, 猪
序列长度 :
591
特异性 :
Recognizes endogenous levels of Paxillin protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学)
应用笔记 :
免疫印迹(1/500 - 1/1000), 免疫组化(1/100 - 1/200), IF(1/100 - 1/500), 免疫细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Paxillin. The exact sequence is proprietary.
产品描述 :
Rabbit polyclonal antibody to Paxillin
NCBI GI登录号 :
170932514
NCBI登录号 :
NP_001074324.1
NCBI基因登录号 :
NM_001080855.2
UniProt数据库登录号 :
P49023
NCBI分子量 :
46,604 Da
NCBI信号通路 :
Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Signaling Events Pathway (138034); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CXCR4-mediated Signaling Events Pathway (137910); Cell Junction Organization Pathway (1270232); Cell-Cell Communication Pathway (1270231); Cell-extracellular Matrix Interactions Pathway (1270237); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864)
NCBI总结 :
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
UniProt数据库总结 :
PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 12q24.31. Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol. Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding. Biological Process: lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; focal adhesion formation; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; cell adhesion
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。