产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗SMAD家族成员2(pS467)抗体
目录 :
MBS8220276
规格 :
0.03毫升
价格 :
165美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 鸡, 牛, 斑马鱼
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法
更多信息或购买 :
图像
图像 1 :
Western blot analysis of SP1 (pT453) expression in Jurkat (A), mouse melanoma cancer (B) whole cell lysates.
图像 2 :
Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
图像 3 :
Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
产品信息
目录号 :
MBS8220276
产品类型 :
抗体
产品全称 :
抗SMAD家族成员2(pS467)抗体
产品简称 :
[SMAD家族成员2]
产品名称同义词 :
[MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2]
其他名称 :
[mothers against decapentaplegic homolog 2 isoform 1; Mothers against decapentaplegic homolog 2; mothers against decapentaplegic homolog 2; SMAD family member 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2]
产品基因名称 :
[SMAD家族成员2]
其他基因名称 :
[SMAD家族成员2;SMAD家族成员2;JV18;MADH2;MADR2;JV18-1;hMAD-2;hSMAD2;MADH2;MADR2;MAD同系物2;母亲抗DPP同系物2;hMAD-2;SMAD 2;SMAD家族成员2;hSMAD2]
UniProt数据库进入名 :
SMAD2_HUMAN
克隆性 :
多克隆
宿主 :
兔
反应物种 :
Human, Mouse, Rabbit, Bovine, Chicken, Monkey, Sheep, Zebrafish, Rat, Pig
序列长度 :
467
特异性 :
Recognizes endogenous levels of SMAD2 (pS467) protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresences (IF), Immunocytochemistry (ICC)
应用笔记 :
酶联免疫吸附测定(1/5000 - 1/10000)。 免疫印迹(1/500 - 1/1000)。 免疫组化(1/100 - 1/200)。 IF(1/100 - 1/500)。 免疫细胞化学(1/100 - 1/500)
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD2 (pS467). The exact sequence is proprietary.
产品描述 :
兔多克隆抗体SMAD家族成员2(pS467)
NCBI GI登录号 :
51173730
NCBI登录号 :
NP_001003652.1
NCBI基因登录号 :
NM_001003652.3
UniProt数据库登录号 :
Q15796
NCBI信号通路 :
AGE/RAGE Pathway (698754); Activin Signaling Pathway (1084757); Activin Signaling Pathway (1108220); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)
NCBI总结 :
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt数据库总结 :
SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 18q21.1. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus. Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor activity; transforming growth factor beta receptor binding. Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; palate development; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; transcription, DNA-dependent; regulation of binding; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; endoderm formation; insulin secretion; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; lung development
尺寸1 :
0.03毫升
价格1 :
165美元
尺寸2 :
0.1毫升
价格2 :
245
size3 :
0.2毫升
价格3 :
350
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。