产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
抗胶原3甲1抗体
目录 :
MBS822102
规格 :
0.03毫升
价格 :
155美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS822102 图像 1
Western blot analysis of Collagen 3 alpha 1 expression in A10 (A), HeLa (B), mouse lung (C), mouse liver (D), rat lung (E) whole cell lysates.
图像 2 :
MyBioSource MBS822102 图像 2
Immunohistochemical analysis of Collagen 3 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
图像 3 :
MyBioSource MBS822102 图像 3
Immunofluorescent analysis of Collagen 3 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
产品信息
目录号 :
MBS822102
产品类型 :
抗体
产品全称 :
抗胶原3甲1抗体
产品简称 :
[胶原3甲1]
产品名称同义词 :
[胶原甲1(III)链]
其他名称 :
[collagen alpha-1(III) chain preproprotein; Collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant; collagen, type III, alpha 1]
产品基因名称 :
[COL3A1]
其他基因名称 :
[COL3A1;COL3A1;EDS4A]
UniProt数据库进入名 :
CO3A1_HUMAN
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠, 牛, 狗, 猪, 兔
序列长度 :
1466
特异性 :
Recognizes endogenous levels of Collagen 3 alpha 1 protein.
纯度 :
The antibody was purified by immunogen affinity chromatography.
形式 :
液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠
储存稳定性 :
Shipping: Blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学)
应用笔记 :
免疫印迹1/500-1/1000。 IH 1/100 - 1/200。 IF/细胞化学1/100-1/500
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其他信息2 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 3 alpha 1. The exact sequence is proprietary.
产品描述 :
兔多克隆抗体胶原3甲1
NCBI GI登录号 :
4502951
NCBI登录号 :
NP_000081.1
NCBI基因登录号 :
NM_000090.3
UniProt数据库登录号 :
P02461
NCBI信号通路 :
Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endothelins Pathway (137958); Extracellular Matrix Organization Pathway (576262)
NCBI总结 :
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
UniProt数据库总结 :
CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Cell adhesion; Extracellular matrix. Chromosomal Location of Human Ortholog: 2q31. Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region; collagen type III. Molecular Function: integrin binding; protein binding; platelet-derived growth factor binding; extracellular matrix structural constituent; metal ion binding; SMAD binding. Biological Process: integrin-mediated signaling pathway; skin development; axon guidance; platelet activation; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; heart development; cell-matrix adhesion; negative regulation of immune response; positive regulation of Rho protein signal transduction; extracellular matrix disassembly; collagen catabolic process; response to radiation; gut development; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; fibril organization and biogenesis; cerebral cortex development; peptide cross-linking; skeletal development; aging. Disease: Ehlers-danlos Syndrome, Type Iv, Autosomal Dominant; Ehlers-danlos Syndrome, Type Iii
尺寸1 :
0.03毫升
价格1 :
155美元
尺寸2 :
0.1毫升
价格2 :
220
size3 :
0.2毫升
价格3 :
295
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。