抗体

抗LATS1/2抗体

MBS8241758

0.03毫升

155美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

抗体

抗LATS1/2抗体

[LATS1/2]

[LATS1; WARTS; Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; LATS2; KPM; Serine/threonine-protein kinase LATS2; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm; Warts-like kinase]

[Serine/threonine-protein kinase LATS1; Serine/threonine-protein kinase LATS1; serine/threonine-protein kinase LATS1; large tumor suppressor kinase 1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts]

[LATS1]

[LATS2]

[LATS1; LATS1; wts; WARTS; h-warts]

LATS1_HUMAN

多克隆

兔

人类, 小鼠, 大鼠

1130

Recognizes endogenous levels of LATS1/2 protein.

The antibody was purified by immunogen affinity chromatography.

液体0.42% 钾磷酸盐, 0.87% 氯化钠, pH值7.3, 30% 甘油, 和0.01% 叠氮化钠

Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

免疫印迹(免疫印迹)

Western Blot: (1/500 - 1/1000)

免疫印迹(免疫印迹)

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LATS1/2. The exact sequence is proprietary.

兔多克隆抗体LATS1/2

52783106

O95835.1

O95835

Hippo Signaling Pathway (1084759); Hippo Signaling Pathway (1108222); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Signal Transduction Pathway (1269379); Signaling By Hippo Pathway (1269616)

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene. [provided by RefSeq, Jul 2012]

LATS1: an AGC kinase of the NDR family of kinases. Localized to the mitotic apparatus and specifically phosphorylated during mitosis. A likely tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity, causing G2 arrest. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. Complexes with CDC2 in early mitosis. LATS1-associated CDC2 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated zyxin, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Knockout mice are susceptible to soft-tissue sarcomas and sensitive to chemical carcinogenesis. Human soft tissue sarcomas have downregulated, mutated, and/or hypermethylated LATS1. Transgenic expression blocks anchorage independent growth in culture and tumor growth in xenografts. Two alternatively spliced isoforms of the human proteinhave been described. Protein type: Protein kinase, AGC; Tumor suppressor; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; NDR family. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: cytosol; microtubule organizing center; spindle pole. Molecular Function: ATP binding; magnesium ion binding; protein binding; protein kinase binding; protein serine/threonine kinase activity. Biological Process: cell division; cytoplasmic sequestering of protein; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; hormone-mediated signaling; inner cell mass cell fate commitment; inner cell mass cellular morphogenesis; keratinocyte differentiation; mitosis; negative regulation of cyclin-dependent protein kinase activity; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of actin filament polymerization; regulation of organ growth; regulation of protein complex assembly; sister chromatid segregation

0.03毫升

155美元

0.1毫升

220

0.2毫升

295