抗体

PKG抗体

MBS840764

0.03毫克

150美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 牛

抗体

PKG抗体

[PKG]

[cGKI alpha CGKI cGKI beta cGMP dependent protein kinase 1alpha isozyme cGMP dependent protein kinase 1beta isozyme KGPB PRKG1 PR]

[PRKG1 protein; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, type I; cGMP-dependent protein kinase I; cGKI]

[PKG]

[PRKG1;PRKG1;PKG;cGK;AAT8;cGK1;cGKI;cGK 1;PRKG1B;PRKGR1B;cGKI-乙;cGKI甲;PRKG1B;PRKGR1A;PRKGR1B;cGK 1;cGK1;cGKI]

KGP1_HUMAN

多克隆

IgG

兔

人类, 小鼠, 大鼠, 牛

686

The antibody recognizes 75 and 85 kDa PKG, corresponding to the molecular mass of two PKG isoforms.

亲和纯化

100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. Appearance: Colorless liquid

Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles

免疫印迹(免疫印迹)

The antibody can be used for Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.

试验数据

Immunogen: Synthetic peptide near the N-terminus of human PKG

Handling: The antibody solution should be gently mixed before use.

Background: PKGs are cyclic GMP-dependent protein kinases (also designed cGKs) that are classified into two types, PKGI and PKGII. Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities. Phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility and platelet aggregation. However, recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases.

148342515

ABQ59040

Adaptive Immune System Pathway (1269171); Ca2+ Pathway (1269615); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Gap Junction Pathway (83072); Gap Junction Pathway (483); Hemostasis Pathway (1269340); Immune System Pathway (1269170); Long-term Depression Pathway (83086); Long-term Depression Pathway (497)

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described. Protein type: Protein kinase, AGC; EC 2.7.11.12; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKG family. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol. Molecular Function: protein binding; calcium channel regulator activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding. Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; neuron migration; dendrite development; blood coagulation; signal transduction; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation

0.03毫克

150美元

0.1毫克

305