抗体

胱氨酸-天冬氨酸蛋白酶9抗体

MBS841177

0.1毫克

305美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 牛

抗体

胱氨酸-天冬氨酸蛋白酶9抗体

[胱氨酸-天冬氨酸蛋白酶9]

[CASP-9; EC 3.4.22.62; ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3; Apaf-3]

[caspase-9; Caspase-9; caspase-9; caspase 9, apoptosis-related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3]

[CASP9;CASP9;MCH6;APAF3;APAF-3;PPP1R56;ICE-LAP6;MCH6;CASP-9;APAF-3;ICE-LAP6]

CASP9_HUMAN

多克隆

IgG

兔

人类, 小鼠, 大鼠, 仓鼠, 牛, 兔, 猪

266

This antibody detects proform (~46kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25kDa) caspase-9

亲和纯化

0.5毫克/毫升亲和纯化兔多克隆在磷酸盐缓冲液中的抗体(PBS), pH值7.2, 含30% 甘油, 0.5% BSA, 5mM乙二胺四乙酸和0.03% ProClin防腐剂

Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

免疫印迹(免疫印迹)

Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide is available seperately ( MBS843499 )

试验数据

Immunogen: Synthetic peptide surrounding amino acid 290 of human caspase-9

Handling: The antibody solution should be gently mixed before use.

Background: Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.

6518502

BAA87905.1

36,564 Da

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms. Protein type: EC 3.4.22.62; Protease; Apoptosis. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: mitochondrion; nucleus; cytosol; apoptosome. Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging

0.1毫克

305美元