抗体

eNOS(磷酸化Ser615)抗体

MBS852269

0.1毫克

215美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

eNOS(磷酸化Ser615)抗体

eNOS

Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS

nitric oxide synthase, endothelial isoform 1; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

eNOS

内皮细胞一氧化氮合酶;内皮细胞一氧化氮合酶;eNOS;ECNOS;cNOS;eNOS;NOSIII

NOS3_HUMAN

兔

人类, 小鼠, 大鼠

1203

eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

WB: 1:500~1:1000, ELISA: 1:40000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S). All Sites: Human: Ser615; Mouse: Ser614; Rat: Ser614. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). Janssens S.P., J. Biol. Chem. 267:22694-22694(1992). Marsden P.A., FEBS Lett. 307:287-293(1992).

40254422

NP_000594.2

NM_000603.4

P29474

67,876 Da

ACE Inhibitor Pathway (198763); AGE/RAGE Pathway (698754); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764)

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol. Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding. Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; regulation of blood pressure; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; regulation of blood vessel size; nitric oxide biosynthetic process; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia. Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic

0.1毫克

215美元