抗体

Smad3(磷酸化Thr179)抗体

MBS858478

0.1毫克

215美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

Smad3(磷酸化Thr179)抗体

Smad3

JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3

mothers against decapentaplegic homolog 3 isoform 2; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; SMAD family member 3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Smad3

SMAD3;SMAD3;LDS3;LDS1C;MADH3;JV15-2;HSPC193;HsT17436;MADH3;MAD同系物3;Mad3;母亲抗DPP同系物3;hMAD-3;SMAD 3;Smad3;hSMAD3

SMAD3_HUMAN

多克隆

IgG

兔

人类, 小鼠, 大鼠

320

Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

免疫印迹(免疫印迹), 免疫组化(免疫组化), 酶联免疫吸附测定(EIA)

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P). All Sites: Human: Thr179; Mouse: Thr179; Rat: Thr179

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

223029440

NP_001138574.1

NM_001145102.1

P84022

25,722 Da

AGE/RAGE Pathway (698754); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adipogenesis Pathway (198832); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809)

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 15q22.33. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex. Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transforming growth factor beta receptor binding. Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; immune response; gene expression; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration. Disease: Loeys-dietz Syndrome 3

0.1毫克

215美元