酶联免疫吸附测定, 免疫组化, 酶免疫法
IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor
insulin-like growth factor-binding protein 3 isoform b; Insulin-like growth factor-binding protein 3; insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex; insulin-like growth factor binding protein 3
IGFBP-3 (Phospho-Ser183) antibody detects endogenous levels of IGFBP-3 only when phosphorylated at serine 183.
ntibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20 degree C for 1 year
IHC: 1:50~1:100, ELISA: 1:1000
Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 90%)
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IGFBP-3 around the phosphorylation site of serine 183 (K-D-SP-Q-R). All Sites: Human: Ser183; Mouse: Ser183
Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
C. Schmid, Am J Physiol Endocrinol Metab, Aug 1994; 267: E226 - E233. Hanh Le, Am J Physiol Endocrinol Metab, May 2006; 290: E952 - E960. C. A. Gibson, J Anim Sci, Jun 1999; 77: 1547 - 1557.
NCBI GI登录号 :
Direct P53 Effectors Pathway (137939); Integrated Pancreatic Cancer Pathway (711360); Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Senescence And Autophagy Pathway (198780); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); P53 Signaling Pathway (83055); P53 Signaling Pathway (465)
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7p12.3. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus. Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; metal ion binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity. Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; positive regulation of MAPKKK cascade; negative regulation of protein amino acid phosphorylation; regulation of cell growth
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