抗体

Phospho-RAF1-S259 Polyclonal Antibody

MBS9128987

0.05毫升

235美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 免疫沉淀

抗体

Phospho-RAF1-S259 Polyclonal Antibody

[磷酸化RAF1]

[RAF1; CMD1NN; CRAF; NS5; Raf-1; c-Raf; Raf-1 p roto-oncogene, serine/threonine kinase]

[RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1 proto-oncogene, serine/threonine kinase; Proto-oncogene c-RAF; cRaf; Raf-1]

[RAF1-S259]

[RAF1; RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF; cRaf]

RAF1_HUMAN

多克隆

IgG

兔

人类, 小鼠

648

亲和纯化

PBS with 50% glycerol, pH7.3.

Store at -20 degree C. Avoid freeze / thaw cycles.

免疫印迹(免疫印迹), 免疫沉淀(免疫沉淀)

免疫印迹1:500-1:2000。 免疫沉淀1:50-1:100

免疫印迹(免疫印迹)

免疫沉淀(IP0

Immunogen: A synthetic phosphorylated peptide around S259 of human RAF1 (NP_002871.1).

磷酸化

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

4506401

NP_002871.1

NM_002880.3

P04049

Observed MW: 73kDa. Calculated MW: 73kDa/75kDa

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (1269471); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1268798); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Apoptosis Pathway (83060); Apoptosis Pathway (470)

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

0.05毫升

235美元

0.1毫升

350

0.2毫升

525