ELISA/assay

Rat Neurofilament light polypeptide, NEFL ELISA Kit

MBS913456

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Rat Neurofilament light polypeptide, NEFL ELISA Kit

neurofilament, light polypeptide

Rat Neurofilament light polypeptide (NEFL) ELISA kit; CMT1F; CMT2E; FLJ53642; NF-L; NF68; NFL; light molecular weight neurofilament protein; neurofilament protein; light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament; lig; neurofilament; light polypeptide

neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; 68 kDa neurofilament protein; neurofilament triplet L protein; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein

NEFL

Nefl; Nefl; Nfl; NF-L; Nf68; Nfl; NF-L

NFL_RAT

大鼠

542

This assay has high sensitivity and excellent specificity for detection of rat NEFL. No significant cross-reactivity or interference between rat NEFL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.81 pg/ml-500 pg/ml. Sensitivity: The minimum detectable dose of rat NEFL is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEFL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEFL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEFL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEFL bound in the initial step. The color development is stopped and the intensity of the color is measured.

13929098

NP_113971.1

NM_031783.1

P19527

61,335 Da

肌萎缩性侧索硬化症 (ALS)通路(83491);肌萎缩性侧索硬化症 (ALS)通路(511)

one component of the hamartin-tuberin tuberous sclerosis complex; may play a role in in central nervous system function [RGD, Feb 2006]

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Cellular Component: TSC1-TSC2 complex; neuron projection; growth cone; axon; cytoplasm; neurofilament; intermediate filament; myelin sheath. Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; phospholipase binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; intermediate filament bundle assembly; microtubule cytoskeleton organization and biogenesis; response to organic nitrogen; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; response to organic substance; intermediate filament polymerization and/or depolymerization; axon transport of mitochondrion; neurofilament cytoskeleton organization and biogenesis; spinal cord development; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800