ELISA/assay

Human Chordin-like protein 1, CHRDL1 ELISA Kit

MBS913563

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Chordin-like protein 1, CHRDL1 ELISA Kit

chordin-like 1

Human Chordin-like protein 1 (CHRDL1) ELISA kit; RP6-141H5.1; CHL; NRLN1; VOPT; dA141H5.1; neuralin 1; neurogenesin-1; ventroptin; chordin-like 1

chordin-like protein 1; Chordin-like protein 1; chordin-like protein 1; neuralin-1; ventroptin; neurogenesin-1; likely ortholog of mouse neuralin 1; chordin-like 1; Neuralin-1; Neurogenesin-1; Ventroptin

CHRDL1

CHRDL1;CHRDL1;NRLN1;NRLN1;VOPT

CRDL1_CHICK

人类

456

This assay has high sensitivity and excellent specificity for detection of human CHRDL1. No significant cross-reactivity or interference between human CHRDL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human CHRDL1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRDL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRDL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

45383778

NP_989502.1

NM_204171.1

Q90ZD5

52,399 Da

Function: Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors.1 PublicationManual assertion based on experiment in:Ref.1

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800