ELISA/assay

小鼠乙类型血小板衍生生长因子受体, 血小板衍生的生长因子受体b酶联免疫吸附试剂盒

MBS919047

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

小鼠乙类型血小板衍生生长因子受体, 血小板衍生的生长因子受体b酶联免疫吸附试剂盒

platelet-derived growth factor receptor, beta polypeptide

Mouse Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA kit; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor; beta polypeptide

platelet-derived growth factor receptor beta isoform 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; PDGF beta chain; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; platelet derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b

血小板衍生的生长因子受体b

Pdgfrb; Pdgfrb; Pdgfr; CD140b; PDGFR-1; AI528809; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1

PGFRB_MOUSE

小鼠

1099

This assay has high sensitivity and excellent specificity for detection of mouse PDGFRB. No significant cross-reactivity or interference between mouse PDGFRB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: 5.86 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.

226342982

NP_001139740.1

NM_001146268.1

P05622

122,806 Da

Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signal Transduction Pathway (575396); ESC Pluripotency Pathways (198374); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Gap Junction Pathway (83269)

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; Protein kinase, TK; Kinase, protein; TK group; PDGFR family. Cellular Component: cell surface; focal adhesion; membrane; lysosome; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; nucleus; intrinsic to plasma membrane. Molecular Function: platelet-derived growth factor binding; nucleotide binding; platelet-derived growth factor receptor binding; platelet-derived growth factor receptor activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; protein binding; signal transducer activity; protein-tyrosine kinase activity; platelet-derived growth factor beta-receptor activity; transferase activity, transferring phosphorus-containing groups; phosphoinositide 3-kinase binding; kinase activity; ATP binding; receptor binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of collagen biosynthetic process; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; chemotaxis; signal transduction; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; smooth muscle development; positive regulation of MAP kinase activity; nitrogen compound metabolic process; positive regulation of cell proliferation; tissue homeostasis; hemopoiesis; phosphatidylinositol metabolic process; kidney development; regulation of peptidyl-tyrosine phosphorylation; cell migration; phosphoinositide-mediated signaling; positive regulation of mitosis; adrenal gland development; in utero embryonic development; positive regulation of chemotaxis; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800