platelet-derived growth factor receptor, beta polypeptide
Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA kit; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor; beta polypeptide
platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b
This assay has high sensitivity and excellent specificity for detection of human PDGFRB. No significant cross-reactivity or interference between human PDGFRB and analogues was observed.
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human PDGFRB is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI GI登录号 :
Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signal Transduction Pathway (106385); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Gap Junction Pathway (83072); Gap Junction Pathway (483)
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008]
PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Kinase, protein; EC 220.127.116.11; Oncoprotein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; TK group; PDGFR family. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: lysosomal lumen; focal adhesion; cell surface; membrane; apical plasma membrane; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; nucleus; intrinsic to plasma membrane. Molecular Function: protein binding; platelet activating factor receptor activity; platelet-derived growth factor beta-receptor activity; platelet-derived growth factor binding; protein-tyrosine kinase activity; platelet-derived growth factor receptor binding; phosphoinositide 3-kinase binding; platelet-derived growth factor receptor activity; protein kinase binding; ATP binding; receptor binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; wound healing; response to toxin; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; cardiac myofibril assembly; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; signal transduction; response to estradiol stimulus; smooth muscle development; positive regulation of MAP kinase activity; positive regulation of cell proliferation; tissue homeostasis; phosphatidylinositol metabolic process; regulation of peptidyl-tyrosine phosphorylation; inner ear development; epidermal growth factor receptor signaling pathway; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; response to retinoic acid; positive regulation of mitosis; in utero embryonic development; positive regulation of chemotaxis; adrenal gland development; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; G-protein coupled receptor protein signaling pathway; response to hyperoxia; response to hydrogen peroxide; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; innate immune response; negative regulation of apoptosis; positive regulation of cell migration. Disease: Myeloproliferative Disorder, Chronic, With Eosinophilia; Myofibromatosis, Infantile, 1; Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 4
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