ELISA/assay

人类卵泡抑素相关蛋白4, FSTL4酶联免疫吸附试剂盒

MBS920045

96 Strip Wells

760美元

酶联免疫吸附试剂盒

人类卵泡抑素相关蛋白4, FSTL4酶联免疫吸附试剂盒

follistatin-like 4

Human Follistatin-related protein 4 (FSTL4) ELISA kit; follistatin-like 4

follistatin-related protein 4; Follistatin-related protein 4; follistatin-related protein 4; follistatin-like protein 4; follistatin-like 4; Follistatin-like protein 4

FSTL4

FSTL4;FSTL4;KIAA1061

FSTL4_HUMAN

人类

842

This assay has high sensitivity and excellent specificity for detection of human FSTL4. No significant cross-reactivity or interference between human FSTL4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human hollistatin-related protein 4 (FSTL4) concentrations in serum, plasma, tissue homogenates. Detection Range: 1.56 ng/ml-100 ng/ml.

Sensitivity: The minimum detectable dose of human FSTL4 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSTL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSTL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

54792136

NP_055897.1

NM_015082.1

Q6MZW2

93,096 Da

96 Strip Wells

760美元